Chin J Dent Res 13 (2010), No. 2 31. Dec. 2010
Chin J Dent Res 13 (2010), No. 2 (31.12.2010)
Page 109-114, PubMed:21264360
Combined Association of CCR2-V64I and MCP-1-2518A/G Polymorphisms with Generalised Aggressive Periodontitis in Chinese
Zhu, Xiao Ling / Meng, Huan Xin / Xu, Li / Zhang, Li / Chen, Zhi Bin / Shi, Dong / Feng, Xiang Hui
Objective: To examine the possible association of CCR2-V64I and MCP-1-2518A/G polymorphisms with generalised aggressive periodontitis (GAgP) in the Chinese population.
Methods: One hundred and twenty-four GAgP patients and 94 healthy subjects were included in the study. A peripheral blood sample was obtained from each subject and genomic DNA was isolated. Gene polymorphisms of CCR2-V64I and MCP-1-2518A/G were analysed by standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
Results: A possible combined effect of CCR2-V64I and MCP-1-2518A/G was observed in the female GAgP patients, as the odds ratio for VV genotype (CCR2) and G+ genotype ( MCP-1) was 0.2 (P = 0.023). Individuals carrying VV genotype and G+ genotype were at reduced risk for GAgP. A possible combined effect of genotype and smoking was observed in the male GAgP patients, as the odds ratio for VV genotype (CCR2) and smoking, or G+ genotype (MCP-1) and smoking were 7.4 (P = 0.022) and 4.9 (P = 0.030), respectively.
Conclusion: The combined association of CCR2-V64I and MCP-1-2518A/G polymorphisms may play an important role in determining GAgP susceptibility in Chinese females. A possible combined effect of genotype and smoking on GAgP susceptibility was suggested in males.
Keywords: aggressive periodontitis, CCR2, MCP-1, polymorphism