We are using cookies to implement functions like login, shopping cart or language selection for this website. Furthermore we use Google Analytics to create anonymized statistical reports of the usage which creates Cookies too. You will find more information in our privacy policy.
OK, I agree I do not want Google Analytics-Cookies
Chinese Journal of Dental Research
Chin J Dent Res 10 (2007), No. 2     14. Dec. 2007
Chin J Dent Res 10 (2007), No. 2  (14.12.2007)

Page 33-37


Study of BMP-2 Gene Modified Goat Bone Marrow Stromal Cells in Promoting New Bone Formation
Jiang, Xin Quan / Zhang, Xiu Li / Hu, Jing Zhou / Zhang, Zhi Yuan
Objective: To evaluate the osteoinductive effects of adenoviral encoding human BMP-2 (AdBMP-2) gene modified goat bone marrow stromal cells (bMSCs) in nude mice.
Methods: Primary cultured goat bMSCs were transduced with 50 pfu/ml AdBMP-2 and Ad- LacZ. Gene transfer efficiency was detected by X-gal staining 3 days after AdLacZ gene transduction. AdBMP-2 or AdLacZ gene modified bMSCs were injected into nude mice intramuscularly (number of injection sites: AdBMP-2 = 9; AdLacZ = 9). Three samples in each group were obtained after 2 weeks, and the remaining six were harvested after 4 weeks. All samples were evaluated by histology. MicroCT, BMP-2 immunohistochemistry and X-gal staining were performed for 4-week samples.
Results: Gene transfer efficiency reached above 70%. Two weeks after injection into nude mice. AdBMP-2 samples demonstrated a combination of relatively mature bone formation and a lesser amount of cartilaginous tissue. In contrast, only fibroblastic-like tissue was detected in Ad- LacZ-transduced bMSCs samples. At week 4, all six AdBMP-2 sites had new bone formation, while in the AdLacZ group no obvious radiopaque area was detectable by microCT. Histologically, AdBMP-2 transduction induced a large bone mass with fatty marrow tissue. While the AdLacZ group showed mainly fibrotic tissue proliferation, X-gal staining confirmed the bMSCs origin in the AdLacZ group.
Conclusion: bMSCs modified with AdBMP-2 gene can promote new bone formation in a nude mice model and could be used for further bone regeneration in the oral and maxillofacial region.

Keywords: bone marrow stromal cells, bone morphogenetic protein, gene therapy