We are using cookies to implement functions like login, shopping cart or language selection for this website. Furthermore we use Google Analytics to create anonymized statistical reports of the usage which creates Cookies too. You will find more information in our privacy policy.
OK, I agree I do not want Google Analytics-Cookies
Chinese Journal of Dental Research
Chin J Dent Res 23 (2020), No. 2     16. June 2020
Chin J Dent Res 23 (2020), No. 2  (16.06.2020)

Page 143-150, doi:10.3290/j.cjdr.a44750, PubMed:32548605


Regeneration of Dental Pulp Tissue by Autologous Grafting Stem Cells Derived from Inflammatory Dental Pulp Tissue in Immature Premolars in a Beagle Dog
Ling, Long / Zhao, Yu Ming / Wang, Xiao Tong / Wen, Quan / Ge, Li Hong
Objective: To compare the biological characteristics of dental pulp stem cells (DPSCs) and inflamed dental pulp derived stem cells (I-DPSCs) in vitro and their regeneration potential in Beagle immature premolars.
Methods: Pulpitis was induced in the premolars of one beagle dog by opening the pulp chamber for 2 weeks, and inflammation was histologically confirmed. DPSCs and I-DPSCs were isolated from normal and inflamed dental pulp, and cell morphology, expression of mesenchymal stem cell markers, clone formation ability, cell proliferation and osteogenic/odontogenic differentiation potential were compared. The dental pulp of 20 roots from 10 immature premolars was extracted and divided into two groups. DPSCs or I-DPSCs with scaffolds were transplanted into the root canals. The roots were extracted after 3 months, and pulp regeneration was evaluated by histological analysis. The data were statistically analysed using one-way ANOVA and a Student t test.
Results: Histological analyses showed lymphocyte infiltration and elevated TNF-α expression, which confirmed the diagnosis of pulpitis. I-DPSCs showed similar morphology, marker gene expression and clone formation ability but greater proliferation ability and osteogenic/odontogenic differentiation potential. Pulp-like tissue formation and bone- and dentine-like tissue deposition were observed in both DPSC- and I-DPSC–transplanted roots.
Conclusion: DPSCs derived from inflammatory dental pulp tissue have similar biological characteristics to those from normal dental pulp and could mediate pulp and dentine regeneration in immature premolars.

Keywords: dental pulp stem cells, inflamed dental pulp, tissue regeneration