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Chinese Journal of Dental Research
Chin J Dent Res 19 (2016), No. 2     4. July 2016
Chin J Dent Res 19 (2016), No. 2  (04.07.2016)

Page 103-108, doi:10.3290/j.cjdr.a36180, PubMed:27379348

Osteoprotegerin Promotes Cementoblastic Activity of Murine Cementoblast Cell Line in vitro
Zhang, Ying Ying / Zhao, Hua Xiang / Chen, Zhi Bin / Lin, Jiu Xiang / Liu, Yan
Objective: To investigate the effect of osteoprotegerin (OPG) on the cementoblastic activity of a clonal population of immortalised murine cementoblasts (OCCM-30) in vitro.
Methods: OCCM-30 cells were transiently transfected with the mouse OPG using the Avalanche transfection reagent. The ectopic expression of OPG was confirmed by enzyme-linked immunosorbent assay and quantitative polymerase chain reaction. The cell counting Kit-8 assay was used to investigate the effect of OPG on cell proliferation. The expression levels of cementoblastic-related mRNA and protein in the transfected OCCM-30 cells were detected using real-time PCR, Western blotting and immunohistochemical staining.
Results: Satisfactory transfection efficiency was observed 48 h after transfection. The results of the cell proliferation assay indicated that the expansion rate of the OPG transfection group was greater than that of the control group at both 72 h and 96 h. The mRNA levels of osterix (Osx), protein kinase B (Akt1), cementum attachment protein (CAP) and osteopontin (Opn) were significantly upregulated (P < 0.05) in the OPG group. Protein levels of OPN, bone sialoprotein II (BSP II), osteocalcin (OC) and CAP, which are responsible for osteogenetic and cementoblastic activity, were significantly increased in the OPG-overexpressing group.
Conclusion: Overexpression of OPG in OCCM-30 cells promotes cementoblastic activity.

Keywords: cementoblastic, OCCM-30, osteoprotegerin (OPG), root repair, root resorption