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Chinese Journal of Dental Research
Chin J Dent Res 17 (2014), No. 2     19. Dec. 2014
Chin J Dent Res 17 (2014), No. 2  (19.12.2014)

Page 91-98, doi:10.3290/j.cjdr.a33271, PubMed:25531016

Attenuation of Inflammatory Response by 25- hydroxyvitamin D3-loaded Polylactic Acid Microspheres in Treatment of Periodontitis in Diabetic Rats
Li, Hao / Li, Bo / Wang, Qi / Xiao, Yu / Chen, Xin Min / Li, Wei
Objective: To investigate the inhibitory effects of 25-hydroxyvitamin D3 (25(OH)D3)-loaded polylactic acid (PLA) microspheres on inflammatory response in diabetic periodontitis.
Methods: 25(OH)D3-loaded PLA microspheres were produced using emulsion-solvent evaporation method. Rat bone marrow stromal cells (BMSCs) cultured with Aggregatibacter actinomycetemcomitans in high glucose medium were chosen to mimic diabetic periodontitis. After cultivation with 25(OH)D3-loaded PLA microspheres, inflammation-related proteins in BMSCs were detected using Western blot analysis. Periodontitis was induced using silk ligatures in diabetic rats, and 25(OH)D3-loaded PLA microspheres were placed into the periodontal pockets. Periodontal tissues were examined using hematoxylin and eosin staining and western blot analysis.
Results: Drug release from the 25(OH)D3-loaded microspheres was relatively steady during 70 days. In a diabetic periodontitis-like environment, 25(OH)D3-loaded microspheres upregulated vitamin D receptor expression, and downregulated nuclear factor-κB expression and signal transducer and activator of transcription 3 phosphorylation in the BMSCs. These 25(OH)D3 microspheres also attenuated periodontal inflammatory infiltrate and bone loss in diabetic rats with periodontitis.
Conclusion: 25(OH)D3-loaded microspheres could ameliorate diabetic periodontitis by inhibiting inflammatory response, and may provide a potential therapy for patients with this disease.

Keywords: 25-hydroxyvitamin D3, microsphere, inflammatory response, diabetes, periodontitis