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Chinese Journal of Dental Research
Chin J Dent Res 13 (2010), No. 1     30. June 2010
Chin J Dent Res 13 (2010), No. 1  (30.06.2010)

Page 45-49, PubMed:20936191


Involvement of Notch Signalling Pathway in Senescence of Human Dental Pulp Cells
Zou, Xiao Ying / Zhuang, Heng / Yue, Lin / Gao, Xue Jun
Objective: To evaluate whether the Notch signalling pathway is involved in the senescence of human dental pulp cells.
Methods: Human dental pulp cells were isolated and cultured. The Notch signalling pathway was blocked by adding DAPT (N-[N-(3,5-difluorophenacetyl-L-alanyl)]-S-phenylglycine t-butyl ester, γ-secretase inhibitor, 5 µmol/L) into the culture medium. Cell proliferation was evaluated by MTT assay. Cell senescence was evaluated through expression of senescenceassociated ß-galactosidase (SA-ß-Gal) using ß-galactosidase staining.
Results: The proliferation of the human dental pulp cells decreased dramatically after treatment with DAPT, compared with the control. SA-ß-Gal positive staining cells were seen in the DAPT group, but not in the control group.
Conclusion: The Notch signalling pathway is involved in the senescence of human dental pulp cells.

Keywords: dental pulp cells, senescence, Notch signalling pathway