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Chinese Journal of Dental Research
Chin J Dent Res 10 (2007), No. 1     16. July 2007
Chin J Dent Res 10 (2007), No. 1  (16.07.2007)

Page 14-20

Differentiation of Bone Marrow Mesenchymal Stem Cells Induced by Indirectly Co-cultured Tooth Germ Cells
Zhuang, Heng / Deng, Zhi Hong / Shi, Jun Nan / Yu, Jin Hua / Jiang, Ming / Nie, Xin / Jin, Yan
Objective: To study the differentiation of bone marrow mesenchymal stem cells (BM-MSCs). When co-cuttured with tooth germ cells (TGCs).
Methods: The BM-MSCs were isolated from tibiae and fibula of Sprague-Dawley (SD) rats aseptically and cultured. The fist molar tooth buds were isolated from E17 SD foetus and processed to obtain TGCs. The BM-MSCs were co-cultured with TGCs indirectly. The differentiation potentiality of BM-MSCs and the tooth-forming potentiality of TGCs were assessed.
Results: The BM-MSCs started to express odontogenic markers, dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1) after 7 days co-culture with TGCs. Implants of co-cultured pellets formed osteoid dentin under renal capsules, while control groups self-differentiated into osseous tissue.
Conclusion: BM-MSCs could be differentiated by co-cultured with TGCs into dental tissues, although the molecular mechanism requires further study.

Keywords: co-culture, differentiation, mesenchymal stem cell, tooth germ